Digital holographic microscopy for longitudinal volumetric imaging of growth and treatment response in three-dimensional tumor models

作者：Li Yuyu; Petrovic Ljubica; La Jeffrey; Celli Jonathan P^*; Yelleswarapu Chandra S

来源：Journal of Biomedical Optics, 2014, 19(11): 116001.

DOI：10.1117/1.JBO.19.11.116001

• digital holographic microscopy
• three-dimensional tumor models
• treatment assessment
• therapeutic screening
• longitudinal imaging
• quantitative phase imaging

We report the use of digital holographic microscopy (DHM) as a viable microscopy approach for quantitative, nondestructive longitudinal imaging of in vitro three-dimensional (3-D) tumor models. Following established methods, we prepared 3-D cultures of pancreatic cancer cells in overlay geometry on extracellular matrix beds and obtained digital holograms at multiple time points throughout the duration of growth. The holograms were digitally processed and the unwrapped phase images were obtained to quantify the nodule thickness over time under normal growth and in cultures subject to chemotherapy treatment. In this manner, total nodule volumes are rapidly estimated and demonstrated here to show contrasting time-dependent changes during growth and in response to treatment. This work suggests the utility of DHM to quantify changes in 3-D structure over time and suggests the further development of this approach for time-lapse monitoring of 3-D morphological changes during growth and in response to treatment that would otherwise be impractical to visualize.

• 出版日期2014-11