A first-generation genetic map of the Japanese scallop Patinopecten yessoensis-based AFLP and microsatellite markers

作者：Xu Kefeng^*; Li Qi; Kong Lingfeng; Yu Ruihai

来源：Aquaculture Research, 2008, 40(1): 35-43.

DOI：10.1111/j.1365-2109.2008.02058.x

• AFLP
• linkage map
• microsatellites
• Patinopecten yessoensis

We constructed genetic linkage maps of the Japanese scallop Patinopecten yessoensis using AFLP and microsatellite markers. With 32 AFLP primer combinations, a total of 413 markers (209 from the female parent and 204 from the male parent) segregated in a 1:1 ratio, corresponding to DNA polymorphisms which were heterozygous in one parent and null in the other. Among the six microsatellite markers we used, there were four polymorphic loci. Two segregated in the female parent, and the other two segregated in both parents. In the maternal parent, 161 framework markers were mapped in 20 linkage groups, with a total coverage of 2198.8 cM. In the paternal parent, 166 framework markers established a map with 21 linkage groups, spanning a genome length of 2137.6 cM. The AFLP markers on the maps were randomly distributed with an average spacing between markers of 14.7-15.6 cM. The estimated coverage for the framework maps are 77.9% both for the female and the male. These are the first linkage maps for P. yessoensis, which constitute a basis for further genome studies and provide a useful framework for consensus map construction by adding orthologous anchor markers developed in P. yessoensis.

• 出版日期2008-12-22
• 单位中国海洋大学