科研之友
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摘要
Grass carp reovirus (GCRV) was first isolated in 1983. In China, the virus caused severe hemorrhagic disease with significant losses of fingerling and yearling grass carp, Cyenopharyngodon idellus. Clade analysis of the different GCRV isolates from China indicates there are three distinct branches, which represent genotypes I, II, and III. However, little is known about the physical-chemical and biological characteristics of viruses from these three genotypes. In this study, the morphologic characteristics of JX-0901 (genotype I), HZ08 (genotype II), and Hubei grass carp disease reovirus (genotype III) were determined using electron microscopy which revealed morphological similarities but viral particles of the isolates arrayed in different ways. A comparison of selected viruses was performed for physical and chemical properties, including stability under different pH conditions, treatment with ether and trypsin, and repeated freeze-thaw cycles. Three isolates showed similar resistance to treatment with ether or trypsin and were stable between pH 3 and pH 10. The viruses exhibited different proliferation curves in the Grass carp swimming bladder cell. No postinfection mortalities or histological lesions were observed in infected rare minnow. Furthermore, cross-protection assays revealed low cross protection between different genotype viruses. This study contributes to the body of knowledge concerning disease control and vaccine development for grass carp hemorrhagic disease.
- 出版日期2016-12
- 单位上海海洋大学; 中国水产科学研究院珠江水产研究所
