The Preconditioning of Berberine Suppresses Hydrogen Peroxide-Induced Premature Senescence via Regulation of Sirtuin 1

作者：Zhu, Xiaofei^*; Yue, Haodi; Guo, Xiaofang; Yang, Jingyi; Liu, Jingshuo; Liu, Jiangtao; Wang, Ruijie; Zhu, Wenjuan

来源：Oxidative Medicine and Cellular Longevity, 2017, 2017: 2391820.

DOI：10.1155/2017/2391820

• MITOCHONDRIAL OXIDATIVE STRESS
• ACTIVATED PROTEIN-KINASE
• CALORIE RESTRICTION
• CELLULAR SENESCENCE
• LIVING CELLS
• METABOLISM
• PROLIFERATION
• LOCALIZATION
• MITOHORMESIS
• RESVERATROL

With a long history of application in Chinese traditional medicine, berberine (BBR) was reported to exhibit healthspan-extending properties in some age-related diseases, such as type 2 diabetes and atherosclerosis. However, the antiaging mechanism of BBR is not completely clear. By means of hydrogen peroxide-(H2O2-) induced premature cellular senescence model, we found that a lowconcentration preconditioning of BBR could resist premature senescence in human diploid fibroblasts (HDFs) measured by senescence-associated beta-galactosidase (SA-beta-gal), accompanied by a decrease in loss of mitochondrial membrane potential and production of intracellular reactive oxygen species (ROS). Moreover, the low-concentration preconditioning of BBR could make cells less susceptible to subsequent H2O2-induced cell cycle arrest and growth inhibition. Experimental results further showed that the low concentration of BBR could induce a slight increase of ROS and upregulate the expression level of sirtuin 1 (SIRT1), an important longevity regulator. H2O2-induced activation of checkpoint kinase 2 (Chk2) was significantly attenuated after the preconditioning of BBR. The present findings implied that the low-concentration preconditioning of BBR could have a mitohormetic effect against cellular senescence triggered by oxidative stress in some age-related diseases through the regulation of SIRT1.

• 出版日期2017
• 单位新乡医学院