MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG

作者：Li, J. J.; Chan, W. H.; Leung, W. Y.; Wang, Y.; Xu, C. S.^*

来源：Genetics and Molecular Research, 2015, 14(2): 4150-4160.

DOI：10.4238/2015.April.27.30

• Apoptosis
• Cell proliferation
• FAS ligand (FASLG)
• Liver regeneration
• MicroRNA-21 (miR-21)

Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequencing. Their role toward rat normal hepatocyte line BRL-3A was studied by gain-and loss-of-function analyses, and one of them, microRNA-21 (miR-21), obviously upregulated and promoted BRL-3A cell proliferation. Using bioinformatics to search for miR-21 targets revealed that Fas ligand (FASLG) is one of miR-21's target genes. A dual-luciferase report assay and Western blot assay showed that miR-21 directly targeted the 3'-untranslated region of FASLG and inhibited the expression of FASLG, which suggests that miR-21 promoted BRL-3A cell proliferation by reducing FASLG expression.

• 出版日期2015
• 单位河南师范大学; 暨南大学