A Comparative Study of the Photosensitizer Penetration into Artificial Caries Lesions in Dentin Measured by the Confocal Raman Microscopy

作者：Melo Mary Anne S; Rolim Juliana P M L; Zanin Iriana C J; Silva Jose J A; Paschoal Alexandre R; Ayala Alejandro P; Rodrigues Lidiany K A^*

来源：Photochemistry and Photobiology, 2014, 90(1): 183-188.

DOI：10.1111/php.12186

This study utilized the confocal Raman microspectroscopy (CRM) technique for the first time to investigate the degree of the penetration of toluidine blue-orto (TBO) in artificial caries lesions produced by two distinct caries-inducing models. The dentin specimens (n=10) were divided into three groups: control, in vitro and in situ. Thereafter, the lesion depth and the demineralization level were evaluated by cross-sectional microhardness (CSMH). CRM mapping across the dentin surface was assessed after the dye application. The CSMH and CRM data were analyzed by t-test and ANOVA, respectively (P<0.05). The values of the lesion depth and the demineralization areas were higher for in situ samples (P<0.05). The TBO penetration values (m) for the control, in vitro and in situ groups were 44.8 +/- 5.6, 46.1 +/- 4.5 and 51.2 +/- 8.5, respectively. There were no statistically significant differences among the groups (P>0.05). The rate of TBO penetration was detected up to about <50m and the demineralization level did not influence the results. These results have showed promising parameters to develop new protocols for deep caries lesions management using photodynamic antimicrobial chemotherapy.

• 出版日期2014-1