Antiangiogenic Activity of Sterically Stabilized Liposomes Containing Paclitaxel (SSL-PTX): In Vitro and In Vivo

作者：Huang, Yue; Chen, Xiao-Mei; Zhao, Bing-Xiang; Ke, Xi-Yu; Zhao, Bo-Jun; Zhao, Xin; Wang, Ying; Zhang, Xuan^*; Zhang, Qiang

来源：AAPS PharmSciTech, 2010, 11(2): 752-759.

DOI：10.1208/s12249-010-9430-z

• antiangiogenic activity
• migration
• paclitaxel
• proliferation
• sterically stabilized liposomes

The purpose of this present study was to evaluate the antiangiogenic activity of sterically stabilized liposomes containing paclitaxel (SSL-PTX). The SSL-PTX was prepared by the thin-film method. The release of paclitaxel from SSL-PTX was analyzed using a dialysis method. The effect of SSL-PTX on endothelial cell proliferation and migration was investigated in vitro. The antitumor and antiangiogenic activity of SSL-PTX was evaluated in MDA-MB-231 tumor xenograft growth in BALB/c nude mice. The release of paclitaxel from SSL-PTX was 22% within 24 h. Our in vitro results indicated that SSL-PTX could effectively inhibit the endothelial cell proliferation and migration at a concentration-dependent manner. We also observed that metronomic SSL-PTX induced marked tumor growth inhibition in MDA-MB-231 xenograft model via the antiangiogenic mechanism, unlike that in paclitaxel injection (Taxol) formulated in Cremophor EL (CrEL). Overall, our results suggested that metronomic chemotherapy with low-dose, CrEL-free SSL-PTX should be feasible and effective.

• 出版日期2010-6
• 单位北京大学; 天然药物与仿生药物国家重点实验室