Altered Expression of Tight Junction Proteins in Cyclosporine Nephrotoxicity

作者：Lee Chang Hwa; Kim Sua; Kang Chong Myung; Kim Wan Young; Kim Jin; Kim Gheun Ho^*

来源：American Journal of Nephrology, 2011, 33(1): 7-16.

DOI：10.1159/000322445

• Cyclosporine nephrotoxicity
• Paracellular permeability
• Sodium chloride symporter
• Tight junction proteins
• WNK4 protein

Background/Aims: The increased permeability of chloride in the distal cortical nephron in cyclosporine nephrotoxicity may involve the transcellular pathway mediated by the thiazide-sensitive Na(+)-Cl(-) cotransporter and/or the paracellular pathway mediated by the tight junctions (TJs). Methods: Cyclosporine was subcutaneously administered to Sprague-Dawley rats for 6 (7.5 mg/kg body weight) and 2 (25 mg/kg body weight) weeks, and immunoblot analysis and immunohistochemistry were carried out from the kidneys. Electrically tight epithelial Madin-Darby canine kidney (MDCK) I cells were exposed to cyclosporine for 72 h to measure changes in transepithelial electrical resistance (Delta TER). Results: Cyclosporine treatment induced a decrease in Na(+)-Cl(-) cotransporter in rat renal cortex. WNK4 protein was increased in both rat kidneys and MDCK I cells. Occludin was also increased in rat kidneys and MDCK I cells exposed to 100 ng/ml cyclosporine. In contrast, cyclosporine treatment induced a decrease in zonula occludens 1 protein abundance and no changes in claudin-1 and claudin-4 in both rat kidneys and MDCK I cells. As a measure of the barrier to small ions, Delta TER of MDCK monolayers was decreased by 100 ng/ml cyclosporine. Conclusion: Renal TJ proteins are affected by cyclosporine treatment. Changes in TJ protein assembly induced by altered expression of WNK4, occludin, and zonula occludens 1 may affect paracellular permeability.

• 出版日期2011