Elucidation of IgE-binding epitopes of Ani s 1: The major Anisakis simplex allergen

作者：Kobayashi Yukihiro; Ikeda Kaori; Shiomi Kazuo^*

来源：Molecular and Biochemical Parasitology, 2010, 174(2): 128-131.

DOI：10.1016/j.molbiopara.2010.07.005

• Anisakis simplex
• Allergen
• Ani s 1
• IgE-binding epitope

Ani s 1, the major allergen of Anisakis simplex, is expected to be a useful antigen in allergen-specific immunotherapy of Anisakis allergy To avoid side-effects such as anaphylactic shock in immunotherapy. It is desirable to use not native allergens but hypoallergenic mutants devoid of IgE-binding epitopes This study was hence aimed to elucidate IgE-binding epitopes of Am Si toward the development of hypoallergenic Ani s 1 mutants Mapping experiments using 32 peptides (P1-32) revealed that major IgE-binding epitopes are included in P24 (region 116-130) and P28 (region 136-150) Furthermore, Ala-scanning and truncation experiments established that eight (underlined in the sequence 116-ELFAREYEGVCKSGK-130) and nine amino acid residues (underlined in the sequence 136-RGSGWMMTILGKSCD-150) are crucial for the IgE-binding of P24 and P28

• 出版日期2010-12