Proteomics of cryoprotective dehydration in Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola)

作者：Thorne M A S^*; Worland M R; Feret R; Deery M J; Lilley K S; Clark M S

来源：Insect Molecular Biology, 2011, 20(3): 303-310.

DOI：10.1111/j.1365-2583.2010.01062.x

• cold tolerance
• DiGE
• LC-MS
• MS
• Onychiurus arcticus
• springtail

The Arctic springtail, Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola), is one of the few organisms known to survive the extreme stresses of its environment by using cryoprotective dehydration. We have undertaken a proteomics study comparing M. arctica, acclimated at -2 degrees C, the temperature known to induce the production of the anhydroprotectant trehalose in this species, and -6 degrees C, the temperature at which trehalose expression plateaus, against control animals acclimated at +5 degrees C. Using difference gel electrophoresis, and liquid chromatography tandem mass spectrometry, we identified three categories of differentially expressed proteins with specific functions, up-regulated in both the -2 degrees C and -6 degrees C animals, that were involved in metabolism, membrane transport and protein folding. Proteins involved in cytoskeleton organisation were only up-regulated in the -6 degrees C animals.

• 出版日期2011-6
• 单位British Antarctic Survey