科研之友
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摘要
In this study, we developed a new magnetically immobilized edible fungus for the biotransformation of panax notoginseng saponins Rbl to Rd and Rg3. The optimum biotransformation conditions were as follows: temperature 32 degrees C, pH 6.5, time 48 h and liquid solid ratio 25:1 (mL/g). The yields of Rd and Rg3 reached 41.35 +/- 0.12 mg/g and 6.35 +/- 0.08 mg/g which increased 6.97-fold and 3.23-fold to that of untreated control, respectively. Additionally, SEM demonstrated that vestured pits and cell walls of samples were destroyed obviously which was beneficial to the Rbl biotransformation and target Rd and Rg3 release. Meanwhile, the reusability of magnetically immobilized microorganism was tested and the activity of the magnetically immobilized microorganism remained 83.8% after 15 runs. The recycling experiments demonstrated that magnetically immobilized fungus exhibited higher efficiency than the non-magnetical one. These results proved that this new magnetically immobilized microorganism could be applied for industrial production and pharmaceutical industry with good efficiency.
