The Genomic Signature of Splicing-Coupled Selection Differs between Long and Short Introns

作者：Farlow Ashley; Dolezal Marlies; Hua Liushuai; Schloetterer Christian

来源：Molecular Biology and Evolution, 2012, 29(1): 21-24.

DOI：10.1093/molbev/msr201

• genome evolution
• intron length
• selection

Understanding the function of noncoding regions in the genome, such as introns, is of central importance to evolutionary biology. One approach is to assay for the targets of natural selection. On one hand, the sequence of introns, especially short introns, appears to evolve in an almost neutral manner. Whereas on the other hand, a large proportion of intronic sequence is under selective constraint. This discrepancy is largely dependent on intron length and differences in the methods used to infer selection. We have used a method based on DNA strand asymmetery that does not require comparison with any putatively neutrally evolving sequence, nor sequence conservation between species, to detect selection within introns. The strongest signal we identify is associated with short introns. This signal comes from a family of motifs that could act as cryptic 5' splice sites during mRNA processing, suggesting a mechanistic justification underlying this signal of selection. Together with an analysis of intron length and splice site strength, we observe that the genomic signature of splicing-coupled selection differs between long and short introns.

• 出版日期2012-1