Design of Asymmetric Peptide Bilayer Membranes

作者：Li Sha; Mehta Anil K^*; Sidorov Anton N; Orlando Thomas M; Jiang Zhigang; Anthony Neil R; Lynn David G^*

来源：Journal of the American Chemical Society, 2016, 138(10): 3579-3586.

DOI：10.1021/jacs.6b00977

Energetic insights emerging from the structural characterization of peptide cross-beta assemblies have enabled the design and construction of robust asymmetric bilayer peptide membranes. Two peptides differing only in their N-terminal residue, phosphotyrosine vs lysine, coassemble as stacks of antiparallel beta-sheets with precisely patterned charged lattices stabilizing the bilayer leaflet interface. Either homogeneous or mixed leaflet composition is possible, and both create nanotubes with dense negative external and positive internal solvent exposed surfaces. Cross-seeding peptide solutions with a preassembled peptide nanotube seed leads to domains of different leaflet architecture within single nanotubes. Architectural control over these cross-beta assemblies, both across the bilayer membrane and along the nanotube length, provides access to highly ordered asymmetric membranes for the further construction of functional mesoscale assemblies.

• 出版日期2016-3-16