Display of Bombyx mori Nucleopolyhedrovirus GP64 on the Bacillus subtilis Spore Coat

作者：Li, Guohui; Tang, Qi; Chen, Huiqing; Yao, Qin; Ning, Degang; Chen, Keping^*

来源：Current Microbiology, 2011, 62(5): 1368-1373.

DOI：10.1007/s00284-011-9867-7

• BACULOVIRUS
• GLYCOPROTEIN
• ANTIGENS
• PROTEIN
• VIRUS

To investigate whether Bombyx mori immunized with Bacillus subtilis spore displaying GP64 escape from the B. mori nucleopolyhedrovirus (BmNPV) attack, a recombinant integrative plasmid named pJS700-GP64 was constructed, which carries a recombinant cotC-Gp64 gene under the control of the cotC promoter. In this study, pJS700-GP64 was transformed into B. subtilis 168 (trp(-)) competent cells, an amylase (amyE) inactivated mutant was selected, and was confirmed to be a double cross-over integrant, cotC-Gp64 fragment of which was integrated into B. subtilis chromosome. Gp64 was expressed on the spore surface and recognized by Gp64-specific antibody. Results of B. mori when challenged with BmNPV indicated that B. mori vaccinated with the recombinant spores possessed resistance to the invasion of BmNPV at some degree.

• 出版日期2011-5
• 单位江苏大学