Dependence of surface monoclonal antibody binding on dynamic changes in Fc gamma RIIb expression

作者：Walker Jennifer A^*; Smith Kenneth G C

来源：Immunology, 2008, 124(3): 412-418.

DOI：10.1111/j.1365-2567.2007.02791.x

• B cell
• CD22
• Fc gamma RIIb
• interleukin-4
• therapeutic antibody

Receptors for the Fc region of immunoglobulin G (Fc gamma Rs) are expressed on a broad range of haematopoietic cell types and are responsible for regulating antibody production and linking the humoral and effector responses. In response to a number of stimuli, such as cytokine signals or inflammation, Fc gamma R expression at the cell surface is dynamically regulated. On B cells, we observed what appeared to be a correlation between CD22 expression and Fc gamma RIIb expression when the latter was varied in a number of models. Further investigation revealed that this was specific to a particular anti-CD22 monoclonal antibody, which appeared to require stabilization by interaction with Fc gamma RIIb for optimal binding to CD22. Since alterations in the regulation of Fc gamma R expression are important in controlling immune responses and have been associated with a number of immune-mediated disease states, we suggest that it might be prudent to confirm the expression of cell surface markers by two independent methods. Furthermore, because the efficacy of therapeutic antibodies may depend upon their interaction with Fc gamma Rs, our results are relevant to their design and assessment.

• 出版日期2008-7
• 单位河北医科大学