Identification and expression of caspase-1 gene under heat stress in insecticide-susceptible and -resistant Plutella xylostella (Lepidoptera: Plutellidae)

作者：Zhuang, Hua Mei; Wang, Kuan Fu; Miyata, Tadashi; Wu, Zu Jian; Wu, Gang^*; Xie, Lian Hui

来源：Molecular Biology Reports, 2011, 38(4): 2529-2539.

DOI：10.1007/s11033-010-0391-9

• Px-caspase-1
• Plutella xylostella
• QPCR expression
• Caspase-3 activity
• Heat stress

A caspase gene in Plutella xylostella (DBM) was identified firstly and named Px-caspase-1. It had a full-length of 1172 bp and contained 900 bp open reading frame that encoded 300 amino acids with 33.6 kDa. The deduced amino acid of Px-caspase-1 had two domain profile including caspase_p20 (position 61-184) and caspase_p10 (position 203-298) (i.e. the big and small catalytic domains), and the highly conserved pentapeptide QACQG in caspase_p20 domain (the recognized catalytic site of caspases). Being highly homologous to effector caspase genes in other insect and mammalian species, Px-caspase-1 was thought to be an effector caspase gene. Heat stress could result in significant mortality increase on adult DBM. Px-caspase-1 mRNA expression and caspase-3 enzyme activity (a effector caspase) were elevated with age and heat treatment. And, heat stress facilitated the procession of Px-caspase-1 expression. Significantly higher mRNA transcription levels were found in a chlorpyrifos-resistant DBM strain, as compared to those in insecticide-susceptible DBM. The results indicated that high temperature could significantly promote apoptosis process resulting in an the increased DBM mortality rate, and that insecticide-susceptible DBM had a significantly higher physiological fitness at high temperatures than insecticide-resistant DBM.

• 出版日期2011-4
• 单位福建农林大学