ATF3 and EGR2 gene expression levels in sdLDL-treated macrophages of patients with coronary artery stenosis

作者：Hosseini Fard Sayed R; Khosravi Mohsen; Yarnazari Amaneh; Hassanpour Parisa; Amirfarhangi Abdollah; Tajik Nader; Nourabad Ghahroodi Faezeh; Najafi Mohammad^*

来源：Journal of Laboratory Medicine, 2018, 42(1-2): 23-29.

DOI：10.1515/labmed-2017-0138

• ATF3
• differentiation
• EGR2
• macrophage
• small dense low density lipoprotein (sdLDL)
• stenosis

Background: The metabolism of cholesteryl esters (CEs) is under the control of a gene network in macrophages. Several genes such as ATF3 and EGR2 are related to cholesterol metabolism.
Methods: In this study, the ATF3 and EGR2 gene expression levels were evaluated in differentiated macrophages of subjects undergoing coronary artery angiography [controls (<5% stenosis), patients (>70% stenosis)] after treatment with small dense low density lipoprotein (sdLDL) particles. Monocytes were isolated using a RosetteSep Kit and were differentiated into macrophages using the M-CSF factor. A modified heparin-MgSO4-PEG method was used for the sdLDL preparation. The ATF3 and EGR2 gene expression levels were measured by the real-time quantitative polymerase chain reaction (RT-qPCR) technique.
Results: In contrast with the control group (p = 0.4), the ATF3 expression level reduced significantly in the differentiated macrophages from all patients [single vessel disease (SVD), fold change 17 times, p = 0.02; two vessel disease (2VD), fold change 1.5 times, p = 0.05; three vessel disease (3VD), fold change 3.5 times, p = 0.04]. Also, the EGR2 expression level reduced significantly in all groups (p < 0.02). The gene fold changes had no significant differences between the patients (p > 0.8).
Conclusions: We propose that the failure of ATF3 gene expression improves the CE synthesis after sdLDL influx. Furthermore, the reduced EGR2 gene expression level in the sdLDL-treated groups may be a negative factor in cholesterol homeostasis.

• 出版日期2018-2