Ag/Au nanoparticles coated graphene electrochemical sensor for ultrasensitive analysis of carcinoembryonic antigen in clinical immunoassay

作者：Huang, Jiaoling; Tian, Jianniao^*; Zhao, Yanchun; Zhao, Shulin

来源：Sensors and Actuators B: Chemical , 2015, 206: 570-576.

DOI：10.1016/j.snb.2014.09.119

• Graphene
• Ag/Au nanoparticles
• Carcinoembryonic antigen

This study demonstrates a novel approach toward development of advanced immunosensors based on chemically functionalized Ag/Au nanoparticles coated on graphene for the detection of carcinoembryonic antigen (CEA) in clinical immunoassay. Ag/Au nanoparticles were synthesized and then were used as a signal amplifying factor for construction of the "sandwich-type" immunosensor. The Ag/Au nanostructure presents good electrochemical activity in the working potential range, which provides high current response. Under optimal conditions, the proposed method could respond to carcinoembryonic antigen (CEA) with a linear calibration range from 10 to 1.2 x 10(5) pg mL(-1) with a detection limit of 8 pg mL(-1). This new biosensor exhibited high sensitivity and selectivity, and was successfully used in determining the CEA which was added in human serum.

• 出版日期2015-1
• 单位广西师范大学