An in situ formed biodegradable hydrogel for reconstruction of the corneal endothelium

作者：Liang Ye; Liu Wanshun^*; Han Baoqin; Yang Chaozhong; Ma Qun; Song Fulai; Bi Qingqing

来源：Colloids and Surfaces B: Biointerfaces , 2011, 82(1): 1-7.

DOI：10.1016/j.colsurfb.2010.07043

• Alginate
• Hydroxypropylated chitosan
• Self-cross-linking
• Biodegradable hydrogel
• Corneal endothelial cells

Biodegradable hydrogels are important biomaterials for tissue engineering and drug delivery. For the purpose of Corneal regenerative medicine, we describe an in situ formed hydrogel based on a water-soluble derivative of chitosan, hydroxypropyl chitosan (HPCTS), and sodium alginate dialdehyde (SAD). Periodate oxidized alginate rapidly cross-links HPCTS clue to Schiff's base formation between the available aldehyde and amino groups Hydrogel cytotoxicity, degradability and histocompatibility in vivo were examined. The potential of the composite hydrogel for corneal endothelium reconstruction was demonstrated by encapsulating corneal endothelial cells (CECs) to grow on Descemet's membranes The results demonstrate that the composite hydrogel was both non-toxic and biodegradable and that CECs transplanted by the composite hydrogel could survive and retain normal morphology. These results provide an opportunity for corneal endothelium reconstruction based on tissue engineering by the in situ formed composite hydrogel.

• 出版日期2011-1-1
• 单位中国海洋大学