Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-kappa B and AP-1 signaling

作者：Jedinak Andrej; Dudhgaonkar Shailesh; Wu Qing li; Simon James; Sliva Daniel^*

来源：Nutrition Journal, 2011, 10(1): 52.

DOI：10.1186/1475-2891-10-52

Background: Mushrooms are well recognized for their culinary properties as well as for their potency to enhance immune response. In the present study, we evaluated anti-inflammatory properties of an edible oyster mushroom (Pleurotus ostreatus) in vitro and in vivo.
Methods: RAW264.7 murine macrophage cell line and murine splenocytes were incubated with the oyster mushroom concentrate (OMC, 0-100 mu g/ml) in the absence or presence of lipopolysacharide (LPS) or concanavalin A (ConA), respectively. Cell proliferation was determined by MTT assay. Expression of cytokines and proteins was measured by ELISA assay and Western blot analysis, respectively. DNA-binding activity was assayed by the gel-shift analysis. Inflammation in mice was induced by intraperitoneal injection of LPS.
Results: OMC suppressed LPS-induced secretion of tumor necrosis factor-alpha (TNF-alpha, interleukin-6 (IL-6), and IL-12p40 from RAW264.7 macrophages. OMC inhibited LPS-induced production of prostaglandin E2 (PGE(2)) and nitric oxide (NO) through the down-regulation of expression of COX-2 and iNOS, respectively. OMC also inhibited LPS-dependent DNA-binding activity of AP-1 and NF-kappa B in RAW264.7 cells. Oral administration of OMC markedly suppressed secretion of TNF-alpha and IL-6 in mice challenged with LPS in vivo. Anti-inflammatory activity of OMC was confirmed by the inhibition of proliferation and secretion of interferon-gamma (IFN-gamma), IL-2, and IL-6 from concanavalin A (ConA)-stimulated mouse splenocytes.
Conclusions: Our study suggests that oyster mushroom possesses anti-inflammatory activities and could be considered a dietary agent against inflammation. The health benefits of the oyster mushroom warrant further clinical studies.

• 出版日期2011-5-16