A method for the simultaneous extraction and quantitation of lipophilic antioxidants in Triticum sp by HPLC-DAD/FLD-MSn

作者：Ziegler Jochen U; Schweiggert Ralf M^*; Carle Reinhold

来源：Journal of Food Composition and Analysis, 2015, 39: 94-102.

DOI：10.1016/j.jfca.2014.11.011

• Einkom
• High-throughput screening
• Fluorescence detection
• Mass spectrometry
• Cereals
• Food analysis
• Vitamin E
• Phenolic lipids
• Food composition

A method for the simultaneous determination of carotenoids, tocopherols, tocotrienols, alk(en)ylresorcinols, and steryl ferulates from whole grain wheat flour was developed and validated using HPLCDAD/FLD-MSn. After comparing various solvents and extraction techniques, samples were extracted with acetone without previous saponification. Extraction recoveries ranged from 93.2 to 101.6% for carotenoids, tocopherols, and steryl ferulates, while 83.4-99.4% were determined for alkylresorcinols. The inter-day CV for all analytes was below 5%. HPLC separation was compared on porous particle C(18)and C-30- as well as a core-shell particle C-18-column, achieving optimum separation on the latter within 40 min after gradient optimization. The limit of detection (LOD) ranged from 0.03 to 20.41 ng on column for lutein and tricosylresorcinol, respectively. Further validation parameters are reported in detail. HPLC-MSn supported the identification of minor compounds, when standards were unavailable. This method is proved to be suitable for high-throughput screening of a large number of lipophilic compounds in wheat.

• 出版日期2015-5