Detection of bovine papillomavirus type 14 DNA sequences in urinary bladder tumors in cattle

作者：Roperto Sante^*; Munday John S; Corrado Federica; Goria Maria; Roperto Franco

来源：Veterinary Microbiology, 2016, 190: 1-4.

DOI：10.1016/j.vetmic.2016.04.007

• Bovine Deltapapillomavirus
• Bovine papillomavirus type 14 (BPV-14)
• Bladder tumors
• Papillomavirus co-infection

Bovine papillomavirus type 14 (BPV-14) is a novel Deltapapillomavirus (delta PV) which is most closely related to BPV-1, -2, and -13, well-known members of the delta PV genus. So far BPV-14 has been detected in cutaneous neoplastic lesions in cattle and in feline sarcoids. As BPV-14 may share biological and pathological properties with BPV-1, -2 and -13, it has been hypothesized that, like other delta PV5, BPV-14 could be associated with bovine bladder neoplasia.
In this study, 50 tumors of the urinary bladder of cattle were diagnosed. DNA was extracted from all tumor samples as well as from 25 normal bladder samples and submitted to BPV-14 L1 PCR and subsequent amplicon sequencing analysis. BPV-14 L1 DNA sequences of specific 195 bp amplicons were obtained from 17 of 50 (34%) tumor DNA isolates; no BPV-14 DNA was detected from 25 normal samples. Amplicons revealed a 99% homology with the corresponding BPV-14 L1 DNA region (GenBank accession number KP276343.1). Co-infections by two or three delta PV types were also seen. This study reveals the presence of BPV-14 DNA alone or in combination with other delta PV DNA in bovine bladder tumors alone and suggests that BPV-14 could also be involved in bladder neoplasia as its E5 oncoprotein has the potential to induce cell proliferation. Furthermore, this is the first study to show the presence of BPV-14 in Europe, suggesting that BPV-14, like other delta PV5, has a worldwide distribution.

• 出版日期2016-7-15