Modeling the protein partitioning in aqueous polymer two-phase systems: influence of polymer concentration and molecular weight

作者：Lin DQ; Wu YT; Mel LH; Zhu ZQ; Yao SJ^*

来源：Chemical Engineering Science, 2003, 58(13): 2963-2972.

DOI：10.1016/S0009-2509(03)00167-2

• modeling
• thermodynamics process
• extraction
• polymer
• aqueous two-phase systems
• protein partitioning

Aqueous polymer two-phase system provides a powerful method for separation and purification of biomaterials. Among various factors, polymer concentration and polymer molecular weight are essential and have strong impact on the protein partitioning in these systems. Based on the modified Pitzer';s model, a simple expression has been obtained for correlating protein partitioning in aqueous polymer two-phase systems with varying polymer concentration and different polymer molecular weights. Using only one group of parameters for each target protein, the partition coefficients of five proteins (lysozyme, chymotrypsinogen-A, bovine serum albumin, transferrin and catalase) in 16 sets of polyethylene glycol (PEG)/dextran systems were correlated. In addition, partition behavior of lactate dehydrogenase in PEG/hydroxypropyl starch systems were measured, correlated and partially predicted. A comparison of calculated and experimental data indicated that the model provides good correlation and prediction abilities on the protein partitioning in aqueous polymer two-phase systems with a wide range of polymer concentration and molecular weight.

• 出版日期2003-7
• 单位浙江大学