A new ethanolamine phosphate-containing variant of the O-antigen of Shigella flexneri type 4a

作者：Perepelov Andrei V^*; L'vov Vyacheslav L; Liu Bin; Senchenkova Sof'ya N; Shekht Mariya E; Shashkov Alexander S; Feng Lu; Aparin Petr G; Wang Lei; Knirel Yuriy A

来源：Carbohydrate Research, 2009, 344(12): 1588-1591.

DOI：10.1016/j.carres.2009.03.022

• Shigella flexneri
• O-Antigen
• Bacterial polysaccharide structure
• Ethanolamine phosphate

The O-specific polysaccharide (O-antigen) structure of a Shigella flexneri type 4a strain from the Dysentery Reference Laboratory (London, UK) was elucidated in 1978 and its characteristic feature was found to be alpha-D-glucosylation of GlcNAc at position 6, which defines O-factor IV. Our NMR spectroscopic studies of the O-specific polysaccharides of two other strains belonging to S. flexneri type 4a (G1668 from Adelaide, Australia, and 1359 from Moscow, Russia) confirmed the carbohydrate backbone structure but revealed in both strains an additional component, ethanolamine phosphate (EtnP), attached at position 3 of one of the rhamnose residues:
[GRAPHICS]
Phosphorylation has not been hitherto reported in any S. flexneri O-antigen. Reinvestigation of the O-specific polysaccharide of S. flexneri type 4b showed that it is not phosphorylated and confirmed its structure established earlier.

• 出版日期2009-8-17
• 单位南开大学