Morphological and molecular identification of nasopharyngeal bot fly larvae infesting red deer (Cervus elaphus) in Austria

作者：Leitner Natascha; Schwarzmann Laurin; Zittra Carina; Palmieri Nicola; Eigner Barbara; Otranto Domenico; Glawischnig Walter; Fuehrer Hans Peter^*

来源：Parasitology Research, 2016, 115(11): 4417-4422.

DOI：10.1007/s00436-016-5206-z

• Cephenemyia auribarbis
• Cervus elaphus
• Cytochrome oxidase subunit I
• Myiasis
• Oestridae
• Pharyngomyia picta

Nasopharyngeal myiases are caused by larvae of bot flies (Diptera: Oestridae), which have evolved a high specificity for their hosts. Bot flies (n = 916) were collected from 137 (57.6 %) out of 238 red deer (Cervus elaphus) hunted in Vorarlberg and Tyrol (Western Austria). After being stored in 75 % ethanol, larvae were identified to species level and developmental stage using morphological and morphometric keys. Larvae were also molecularly characterized by polymerase chain reaction (PCR) amplification and partial sequencing of the mitochondrial cytochrome oxidase subunit I gene. Morphological and molecular analysis allowed identification of larvae as Cephenemyia auribarbis and Pharyngomyia picta. Genetic variations were also examined within the specimens collected in both geographical locations.

• 出版日期2016-11