Axonal ribosomes and mRNAs associate with fragile X granules in adult rodent and human brains

作者：Akins Michael R^*; Berk Rauch Hanna E; Kwan Kenneth Y; Mitchell Molly E; Shepard Katherine A; Korsak Lulu I T; Stackpole Emily E; Warner Schmidt Jennifer L; Sestan Nenad; Cameron Heather A; Fallon Justin R

来源：Human Molecular Genetics, 2017, 26(1): 192-209.

DOI：10.1093/hmg/ddw381

Local mRNA translation in growing axons allows for rapid and precise regulation of protein expression in response to extrinsic stimuli. However, the role of local translation in mature CNS axons is unknown. Such a mechanism requires the presence of translational machinery and associated mRNAs in circuit-integrated brain axons. Here we use a combination of genetic, quantitative imaging and super-resolution microscopy approaches to show that mature axons in the mammalian brain contain ribosomes, the translational regulator FMRP and a subset of FMRP mRNA targets. This axonal translational machinery is associated with Fragile X granules (FXGs), which are restricted to axons in a stereotyped subset of brain circuits. FXGs and associated axonal translational machinery are present in hippocampus in humans as old as 57 years. This FXG-associated axonal translational machinery is present in adult rats, even when adult neurogenesis is blocked. In contrast, in mouse this machinery is only observed in juvenile hippocampal axons. This differential developmental expression was specific to the hippocampus, as both mice and rats exhibit FXGs in mature axons in the adult olfactory system. Experiments in Fmr1 null mice show that FMRP regulates axonal protein expression but is not required for axonal transport of ribosomes or its target mRNAs. Axonal translational machinery is thus a feature of adult CNS neurons. Regulation of this machinery by FMRP could support complex behaviours in humans throughout life.

• 出版日期2017-1-1
• 单位NIH