科研之友
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摘要
OBJECTIVES Interstitial cystitis (IC) is a painful bladder syndrome associated with urinary frequency and urgency. Elusive cause of IC makes its diagnosis only possible by exclusion in many cases. In this study, we used proteomics for identifying disease-associated proteins in a rat model of chronic bladder irritation.
METHODS Chronic irritation of the rat bladder was caused by a brief (90 seconds) intravesical instillation of 0.2 mL of 0.4 N HCI. Whole bladders were collected at different time points after treatment, snap frozen, and nuclear and cyrosolic protein extracts were obtained. Samples were resolved in standard 2-dimensional (21)) gels stained with an improved Coomasie stain or by differential gel electrophoresis (DIGE). Differentially expressed spots were excised and identified by MALDI-TOF MS/MS. Histologic and Western blot analyses were also performed.
RESULTS Bladder morphology and histologic appearance of bladder sections after HCI treatment reflected hemorrhage, edema, epithetial denudation, detrusor mastocytosis, and eosinophilia. Proteomic analysis of irritated rat bladder revealed marked overexpression of 4 nuclear proteins and marked underexpression of I nuclear protein compared with normal rat bladders. Among these proteins, inflammation-associated calgranulin A (over) and smooth muscle protein-22/transgel in (under) showed opposed expression patterns after bladder irritation.
CONCLUSIONS Presence of mast cells and eosinophils and overexpression of calgranulin A confirm the inflammatory component of HCI-irritated bladder. Altered expression of nuclear proteins is of particular interest because of their possible rote as a prognostic marker in inflammatory bladder disorders. However, more studies are needed before clinical application of these findings can be established.
- 出版日期2008-3
