DETERMINATION OF SEMICARBAZIDE-SENSITIVE AMINE OXIDASE ACTIVITY IN BLOOD PLASMA BY A LIGHT SCATTERING TECHNIQUE

作者：Tan, Xuan; Rang, Wei-Qing; Wang, Yong-Sheng^*; Yang, Hui-Xian; Xue, Jin-Hua; Shi, Lin-Fei; Yang, Hong-Mei; Liu, Lu; Zhou, Bin

来源：Analytical Letters, 2012, 45(18): 2774-2784.

DOI：10.1080/00032719.2012.703738

• Benzaldehyde
• Benzylamine
• 2,4-Dinitrophenylhydrazine
• Light scattering
• Semicarbazide-sensitive amine oxidase

A novel method for the determination of semicarbazide-sensitive amine oxidase (SSAO) activity in blood plasma has been developed. The method was based on the change of light scattering (LS) intensity resulting from the derivative product of the interaction of 2,4-dinitrophenylhydrazine (DNPH) with benzaldehyde produced by catalyzing of SSAO to benzylamine. In Britton-Robinson (B-R) buffer solution, the intensity of system's LS at 514.6 nm was significantly enhanced and was directly proportional to the concentration of benzaldehyde. In this method, SSAO enzyme activity is defined as the concentration of benzaldehyde (nmol) formed per mL plasma per hour. The range of determination of SSAO enzyme activity was 6.40 x 10(-3) -0.340 nmol mL(-1) h(-1) with a detection limit of 1.92 x 10(-3) nmol mL(-1) h(-1). The relative standard deviation was 2.8-4.1% and the average recovery was 67.9% (n = 6).

• 出版日期2012
• 单位南华大学