Antitumor efficacy of argon-helium cryoablation-generated dendritic cell vaccine in glioma

作者：Yin, Zhilin; Lu, Guohui; Xiao, Zhenyong; Liu, Tianzhu; He, Xiaozheng; Wang, Qifu; Lin, Chunnan; Zhang, Shizhong^*

来源：NeuroReport, 2014, 25(4): 199-204.

DOI：10.1097/WNR.0000000000000045

• argon-helium cryoablation
• cytotoxic T cells
• dendritic cells
• glioma

Dendritic cells (DCs) are potent antigen-presenting cells that play a critical role in priming tumor immune responses. We investigated the mechanisms of antitumor efficacy of DCs pulsed with argon-helium-cryotreated glioma cells. There was significant upregulation of maturation markers (CD80, CD86, MHC-I, and MHC-II) in argon-helium freeze-thawed lysate-pulsed DCs. The concentration of interleukin-6 (IL-6), IL-1 beta, tumor necrosis factor-alpha, and IL-12 secreted by lysate-pulsed DCs was increased. The concentration of interferon-gamma secreted by T cells stimulated by lysate-pulsed DCs was increased. The cytotoxicity assay showed that T cells stimulated by lysate-pulsed DCs could kill glioma cells significantly more effectively. Our results suggest that argon-helium freeze-thawed lysate-pulsed DCs in vitro can promote DC maturation and enhance DC antigen-presenting function, and induce cytotoxic T lymphocytes to kill tumor cells. Therefore, the combination of argon-helium cryoablation and DC vaccine may represent a novel treatment method for glioma.

• 出版日期2014-3-5
• 单位南方医科大学