AxyR, an AraC Family Transcriptional Activator of the Xylanase 3 Gene, Requires Xylo-Oligosaccharides as a Cofactor for DNA Binding in Paenibacillus sp Strain W-61

作者：Fukuda Mutsumi; Yoshida Shigeki; Itoh Hiroya; Kato Yoji; Watanabe Seiji; Itoh Yoshifumi; Kamio Yoshiyuki; Kaneko Jun^*

来源：Bioscience Biotechnology and Biochemistry, 2012, 76(5): 1051-1054.

DOI：10.1271/bbb.120165

• xylanase
• Paenibacillus
• co-factor
• xylo-oligosaccharides
• DNA-binding protein

The xylanolytic bacterium Paenibacillus sp. strain W-61 encodes three extracellular xylanase genes, xyn1, xyn3, and xyn5. In this study, we identified a transcriptional activator required for transcription of the xyn3 gene in strain W-61. The activator, AxyR, contained the highly homologous AraC-type DNA binding domain and required xylobiose, xylotriose, or xylotetraose as cofactor for binding to the xyn3 promoter region.

• 出版日期2012-5