Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria

作者：Laraba Imane^*; Botreghda Houda; Abdallah Nora; Bouaicha Oussama; Obanor Friday; Moretti Antonio; Geiser David M; Kim Hye Seon; McCormick Susan P; Proctor Robert H; Kelly Amy C; Ward Todd J; O'Donnell Kerry

来源：Fungal Genetics and Biology, 2017, 103: 34-41.

DOI：10.1016/j.fgb.2017.04.001

• 3-Acetyl-deoxynivalenol
• Culmorin
• Mating type
• Mycotoxins
• Nivalenol
• SSR markers

Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR = 110, n FHB = 30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (rt = 27), Australia (n = 30) and the United States (n = 28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where twothirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8% 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77%) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89% of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.

• 出版日期2017-6