Ultra high performance liquid chromatography-mass spectrometric analysis of oxidized free fatty acids and acylglycerols

作者：Tarvainen Marko^*; Suomela Jukka Pekka; Kallio Heikki

来源：European Journal of Lipid Science and Technology, 2011, 113(4): 409-422.

DOI：10.1002/ejlt.201000454

• HPLC
• Lipidomics
• Lipid oxidation
• Mass spectrometry
• UHPLC

A fast ultra high performance liquid chromatography (UHPLC)-electrospray ionization (ESI)-mass spectrometric (MS) method was developed for simultaneous analysis of free fatty acids (FFAs), monoacylglycerols (MAG), diacylglycerols (DAG), triacylglycerols (TAG), and their oxidized equivalents. Effect of elevated column temperature was studied in order to optimize the chromatography of closely eluting peaks and to reduce high back pressure formed in UHPLC. The elevated temperature enabled high flow rate, better mass transfer, and therefore more narrow peaks and better separation of the analytes. The new method was applied to the analysis of total lipid extracts of lipolysis samples prepared by an artificial digestion model in order to investigate oxidized lipids and changes in their profiles in the chyme. Over 150 compounds were identified from the extracts. The UHPLC-ESI-MS method was proved to be fast, highly selective, and sensitive. Compared to a previously used high performance LC-ESI-MS method, the new UHPLC-ESI-MS method was over five times faster and consumed one tenth of the solvents while producing comparable quantitative results.

• 出版日期2011-4