A large family of Dscam genes with tandemly arrayed 5′ cassettes in Chelicerata

作者：Yue, Yuan; Meng, Yijun; Ma, Hongru; Hou, Shouqing; Cao, Guozheng; Hong, Weiling; Shi, Yang; Guo, Pengjuan; Liu, Baoping; Shi, Feng; Yang, Yun; Jin, Yongfeng^*

来源：Nature Communications, 2016, 7(1): 11252.

DOI：10.1038/ncomms11252

• DENDRITIC SELF-AVOIDANCE
• DROSOPHILA-DSCAM
• AXON GUIDANCE
• MOLECULAR DIVERSITY
• ALPHA
• PROTOCADHERINS
• SUPERFAMILY
• EXPRESSION
• PROMOTER
• IDENTITY

Drosophila Dscam1 (Down Syndrome Cell Adhesion Molecules) and vertebrate clustered protocadherins (Pcdhs) are two classic examples of the extraordinary isoform diversity from a single genomic locus. Dscam1 encodes 38,016 distinct isoforms via mutually exclusive splicing in D. melanogaster, while the vertebrate clustered Pcdhs utilize alternative promoters to generate isoform diversity. Here we reveal a shortened Dscam gene family with tandemly arrayed 50 cassettes in Chelicerata. These cassette repeats generally comprise two or four exons, corresponding to variable Immunoglobulin 7 (Ig7) or Ig7-8 domains of Drosophila Dscam1. Furthermore, extraordinary isoform diversity has been generated through a combination of alternating promoter and alternative splicing. These sDscams have a high sequence similarity with Drosophila Dscam1, and share striking organizational resemblance to the 50 variable regions of vertebrate clustered Pcdhs. Hence, our findings have important implications for understanding the functional similarities between Drosophila Dscam1 and vertebrate Pcdhs, and may provide further mechanistic insights into the regulation of isoform diversity.

• 出版日期2016-4
• 单位浙江大学; 杭州师范大学