A codominant SCAR marker linked to the genic male sterility gene (ms(1)) in chili pepper (Capsicum annuum)

作者：Lee J; Yoon J B^*; Han J H; Lee W P; Do J W; Ryu H; Kim S H; Park H G

来源：Plant Breeding, 2010, 129(1): 35-38.

DOI：10.1111/j.1439-0523.2009.01643.x

• chili pepper
• genic male sterility
• ms(1)
• AFLP-SCAR

As one of the genic male sterility (GMS) materials in chili pepper (Capsicum annuum L.), GMS1 has been used for commercial F-1 hybrid seed production. The male sterility of GMS1 is controlled by a recessive nuclear gene, named ms(1). In this study, we developed DNA markers linked to the ms(1) locus using a combination of bulked segregant analysis and amplified fragment length polymorphism (AFLP) in a segregating sibling population. From the screening of 1024 AFLP primer combinations, the AFLP marker E-AGC/M-GTG (514 bp) was identified as being linked to the ms(1) locus at a distance of about 3 cM. Based on internal sequencing analysis of the E-AGC/M-GTG marker between male fertile and sterile plants, we identified three small deletions with a size of altogether 42 bp in the male-fertile plant and developed a codominant sequence characterized amplified region (SCAR) marker. This SCAR marker may be valuable for marker-assisted breeding in the hybrid seed production system of chili pepper using the GMS1 line.

• 出版日期2010-2