A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo

作者：Wang, Xiaoyi; Min, Jie; Wang, Weijie; Wang, Yan; Yin, Gui^*; Wang, Ruiyong^*

来源：Analyst, 2018, 143(11): 2641-2647.

DOI：10.1039/c8an00586a

• NAKED-EYE DETECTION
• REACTIVE OXYGEN
• SELECTIVE DETECTION
• RATIOMETRIC DETECTION
• RECENT PROGRESS
• CELL-CULTURE
• MITOCHONDRIA
• ACID
• HOCL
• WATER

Reactive oxygen species (ROS), especially HOCl/ClO-, have been demonstrated to play essential roles in both physiological and pathological processes, and an abnormal level of HOCl/ClO- is related to some diseases. In this work, a very fast responsive (within 30 seconds) porphyrin-based fluorescent probe, TPP-TCF, for ClO- with a NIR emissive wavelength was prepared. This probe exhibited excellent selectivity towards ClO- and would not be interfered with by other ROS and typical nucleophiles. The limit of detection (LOD) for ClO- was evaluated to be 0.29 mu M, indicating high sensitivity towards ClO-. In further bioimaging experiments, TPP-TCF displayed low-cytotoxicity and good cell penetrability for recognizing exogenous ClO- in HeLa cells. Moreover, this probe was successfully applied in imaging endogenous ClO- in living animals.

• 出版日期2018-6-7
• 单位医药生物技术国家重点实验室; 生命分析化学国家重点实验室; 南京大学