A high molecular weight protein Bengalin from the Indian black scorpion (Heterometrus bengalensis CL Koch) venom having antiosteoporosis activity in female albino rats

作者：Haldar Subhash; Das Gupta Shubho; Gomes Aparna; Giri Biplab; Dasgupta Subir Chandra; Biswas Ajay; Mishra Roshnara; Gomes Antony

来源：Toxicon, 2010, 55(2-3): 455-461.

DOI：10.1016/j.toxicon.2009.09.013

• Osteoporosis
• Experimental osteoporosis
• Scorpion
• Scorpion venom protein
• Bengalin

This study reports the presence of a high molecular weight protein (Bengalin) from the Indian black scorpion (Heterometrus bengalensis) venom having anti osteoporosis activity in experimental osteoporosis developed in female albino Wister rats. Bengalin was purified through DEAE-cellulose ion exchange chromatography and high performance liquid chromatography. The molecular weight of the Bengalin was found to be 72 kDa and the first 20 amino acid sequence was found to be G-P-L-T-I-L-H-I-N-D-V-H-A-A/R-F-E-Q/G-F/G-N-T. Bengalin exhibited significant antiosteoporosis activity in experimental female rats, 21 which was confirmed through analysis of urine Ca2+, PO43-, CRE & OH-P. Bengalin (3 mu g and 5 mu g/100 g rat/i.p.) antagonized osteoporosis by restoring urinary Ca2+, PO43-, CRE and OH-P, serum/plasma Ca2+, PO43-, ALP, TRAP, PTH, T-3, TSH, Osteocalcin, IL1 IL6 and TNF alpha and bone minerals Ca2+, P, Mg2+, Zn2+, Na+, as compared with the sham operated control rats. Bone minerals density of osteoporosis female rats was improved due to Bengalin, observed through DEXA scan. Subacute toxicity studies in male albino mice, Bengalin showed cardiotoxicity. In vivo experiments, Bengalin showed cardiotoxicity on isolated guinea pig heart, guinea pig auricle, and neurotoxicity on isolated rat phrenic nerve diaphragm preparation. Further detail studies on the toxicity, antiosteoporosis and structural identity of Bengalin are warranted.

• 出版日期2010-3