Determination of nanoparticles concentration by multivariate curve resolution

作者：Hemmateenejad Bahram^*; Safavi Afsaneh; Honarasa Fatemeh

来源：Chemometrics and Intelligent Laboratory Systems, 2015, 141: 88-93.

DOI：10.1016/j.chemolab.2014.12.004

• Nanoparticles concentration
• Spectrophotometry
• Chemometrics
• MCR-ALS

The use of multivariate curve resolution-alternating least square (MCR-ALS) for determination of nanopartides concentration is proposed. The concentration of nanoparticles based on its reaction with a reagent such as a biomolecule, nanoparticles and etc was calculated. The reagent can be considered as an aggregating agent, capping agent and so on. To do so, titration of nanoparticle was achieved by adding variable amounts of reagent to a nanoparticle solution of unknown concentration. A reverse titration was also performed. The UV-Vis spectra of the solutions at different titration steps were recorded. After data collection, chemometrics approach was employed for data analysis. To use MCR-ALS as a quantitative tool, rotational and intensity ambiguities were removed employing unimodality, non-negativity, closure and equality constraints and simultaneous analysis of four spectroscopic titrations: three titrations of reagent by nanoparticles with different concentrations of reagent, and one titration of nanoparticles by reagent. The proposed method was firstly evaluated by simulated data. Then, the concentration of the laboratory-synthesized silver nanoparticles was determined using human serum albumin as reagent. The estimated concentration by the suggested method (1.24 x 10(-8) M) was very close to that obtained by analysis of TEM images (1.19 x 10(-8) M) with a relative error of 4.2%.

• 出版日期2015-2-15