Arctigenin suppresses receptor activator of nuclear factor kappa B ligand (RANKL)-mediated osteoclast differentiation in bone marrow-derived macrophages

作者：Kim A Ram; Kim Hyuk Soon; Lee Jeong Min; Choi Jung Ho; Kim Se Na; Kim Do Kyun; Kim Ji Hyung; Mun Se Hwan; Kim Jie Wan; Jeon Hyun Soo; Kim Young Mi; Choi Wahn Soo^*

来源：European Journal of Pharmacology, 2012, 682(1-3): 29-36.

DOI：10.1016/j.ejphar.2012.02.026

• Arctigenin
• Osteoclast differentiation
• RANKL
• Bone marrow macrophages
• Syk

Osteoclasts, multinucleated bone-resorbing cells, are closely associated with bone diseases such as rheumatoid arthritis and osteoporosis. Osteoclasts are derived from hematopoietic precursor cells, and their differentiation is mediated by two cytokines, including macrophage colony stimulating factor and receptor activator of nuclear factor kappa B ligand (RANKL). Previous studies have shown that arctigenin exhibits an anti-inflammatoly effect. However, the effect of arctigenin on osteoclast differentiation is yet to be elucidated. In this study, we found that arctigenin inhibited RANKL-mediated osteoclast differentiation in bone marrow macrophages in a dose-dependent manner and suppressed RANKL-mediated bone resorption. Additionally, the expression of typical marker proteins, such as NFATc1, c-Fos, TRAF6, c-Src, and cathepsin K, were significantly inhibited. Arctigenin inhibited the phosphorylation of Erk1/2, but not p38 and JNK, in a dose-dependent manner. Arctigenin also dramatically suppressed immunoreceptor tyrosine-based activation motif-mediated costimulatory signaling molecules, including Syk and PLC-gamma 2, and Gab2. Notably, arctigenin inhibited the activation of Syk through RANKL stimulation. Furthermore, arctigenin prevented osteoclast differentiation in the calvarial bone of mice following stimulation with lipopolysaccharide. Our results show that arctigenin inhibits osteoclast differentiation in vitro and in vivo Therefore, arctigenin may be useful for treating rheumatoid arthritis and osteoporosis.

• 出版日期2012-5-5