High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom

作者：Liberato Tarcisio; Paolo Troncone Lanfranco Ranieri; Yamashiro Edson T; Serrano Solange M T; Zelanis Andre^*

来源：Amino Acids, 2016, 48(3): 901-906.

DOI：10.1007/s00726-015-2151-6

• Phoneutria nigriventer
• Proteome
• Spider venom
• Chitinase

Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity.

• 出版日期2016-3