Analysis of Changes in Cell Volume of a Mouse Early Embryo Exposed to Osmotic Shock

作者：Pogorelova M A^*; Goldshtein D V; Pogorelov A G; Golichenkov V A

来源：Bulletin of Experimental Biology and Medicine, 2009, 148(1): 152-154.

DOI：10.1007/s10517-009-0653-5

• two-cell mouse embryo
• osmotic stress
• 3D-reconstruction
• laser scanning microscopy
• cell volume regulation

The impact of the osmotic component of the incubation medium for the volume of mouse early embryonic cell was studied by laser scanning microscopy. Common Dulbecco's medium caused a prolonged hyperosmotic effect. Adaptive phase of regulatory compensation for the osmotic shock was observed under hypotonic conditions. From these data, water permeability of the blastomer membrane is evaluated as 0.4 mu/(minxatm).

• 出版日期2009-7