Detection and Molecular Characterization of Escherichia coli Strains Producers of Extended-Spectrum and CMY-2 Type Beta-Lactamases, Isolated from Turtles in Mexico

作者：Cortes Cortes Gerardo; Lozano Zarain Patricia; Torres Carmen; Castaneda Miguel; Moreno Sanchez Gabriela; Alonso Carla A; Lopez Pliego Liliana; Gutierrez Mayen Maria G; Martinez Laguna Ygnacio; del Carmen Rocha Gracia Rosa

来源：Vector Borne and Zoonotic Diseases, 2016, 16(9): 595-603.

DOI：10.1089/vbz.2014.1725

• CMY-2
• ESBL
• Escherichia coli
• Mexico
• multidrug-resistance
• turtles

Multidrug-resistant bacteria are a growing problem in different environments and hosts, but scarce information exists about their prevalence in reptiles. The aim of this study was to analyze the resistance mechanisms, molecular typing, and plasmid content of cefotaxime-resistant (CTXR) Escherichia coli isolates recovered from cloacal samples of 71 turtles sheltered in a herpetarium in Mexico. CTXR-E. coli were recovered in 11 of 71 samples (15.5%), and one isolate/sample was characterized. Extended-spectrum beta-lactamase (ESBL)-producing E. coli isolates were detected in four samples (5.6%): two strains carried the bla(CTX-M-2) gene (phylogroup D and ST2732) and two contained the bla(CTX-M-15) gene (phylogroup B1 and lineages ST58 and ST156). The bla(CMY-2) gene was detected by PCR in E. coli isolates of eight samples (9.8%) (one of them also carried bla(CTX-M-2)); these isolates were distributed into phylogroups A (n = 1), B1 (n = 6), and D (n = 1) and typed as ST155, ST156, ST2329, and ST2732. Plasmid-mediated quinolone resistance (PMQR) genes were detected in five isolates [aac(6')Ib-cr, qnrA, qnrB19, and oqxB]. From three to five replicon plasmids were detected among the strains, being IncFIB, IncI1, IncF(rep), and IncK the most prevalent. ESBL or pAmpC genes were transferred by conjugation in four strains, and the bla(CTX-M-15) and bla(CMY-2) genes were localized in IncFIB or IncI1 plasmids by Southern blot hybridization assays. Class 1 and/or class 2 integrons were detected in eight strains with six different structures of gene cassette arrays. Nine pulsed-field gel electrophoresis patterns were found among the 11 studied strains. To our knowledge, this is the first detection of ESBL, CMY-2, PMQR, and mobile determinants of antimicrobial resistance in E. coli of turtle origin, highlighting the potential dissemination of multidrug-resistant bacteria from these animals to other environments and hosts, including humans.

• 出版日期2016