An approach to speed up the isolation of hydrophilic metabolites from natural sources at semipreparative level by using a hydrophilic-lipophilic balance/mixed-mode strong cation exchange-high-performance liquid chromatography/mass spectrometry system

作者：Espada Alfonso^*; Anta Cristina; Bragado Aroa; Rodriguez Jaime; Jimenez Carlos

来源：Journal of Chromatography A, 2011, 1218(13): 1790-1794.

DOI：10.1016/j.chroma.2011.01.072

• Hydrophilic-lipophilic balance (HLB)
• Mixed-mode strong cation exchange (MCX)
• SPE
• HPLC/MS
• Small water-soluble compounds
• Natural products
• Siderophore

An approach to speed up the isolation of hydrophilic metabolites in complex natural matrixes by using a HLB/MCX-HPLC/MS system based on the retention properties of hydrophilic-lipophilic and cation exchange polymeric cartridges was developed. This methodology was successfully applied to the reisolation of small water soluble compounds with completely different structures from two different natural extracts such as a dipeptide (vanchrobactin) from a bacterium culture broth and a pyrrolidine bearing a carboxylic acid moiety (clionapyrrolidine A) from a sponge. This method improved not only the efficiency of the isolation methodology but also the isolation time in relation to the existing methods.

• 出版日期2011-4-1