Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID

作者：Zourelidou Melina; Absmanner Birgit; Weller Benjamin; Barbosa Ines C R; Willige Bjoern C; Fastner Astrid; Streit Verena; Port Sarah; Colcombet Jean; van Bentem Sergio de la Fuente; Hirt Heribert; Kuster Bernhard; Schulze Waltraud X; Hammes Ulrich Z^*; Schwechheimer Claus

来源：eLife, 2014, 3: e02860.

DOI：10.7554/eLife.02860

• Arabidopsis
• AGC kinases
• auxin
• auxin efflux
• auxin transport
• protein kinases
• plant

The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the - in many cells - asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.

• 出版日期2014-6-19