High-rate Fermentation of Acetate to Methane under Saline Condition by Aceticlastic Methanogens Immobilized in Marine Sediment

作者：Kita Akihisa; Kobayashi Kounosuke; Miura Toyokazu; Okamura Yoshiko; Aki Tsunehiro; Matsumura Yukihiko; Tajima Takahisa; Nishio Naomichi; Nakashimada Yutaka^*

来源：Journal of the Japan Petroleum Institute, 2016, 59(1): 9-15.

DOI：10.1627/jpi.59.9

• Methane
• Acetate
• Aceticlastic methanogen
• Fixed-bed reactor
• Saline condition

High-rate production of methane from acetate using a fixed-bed reactor filled with marine sediment containing halotolerant aceticlastic methanogens and 3 % NaCl was investigated. In continuous culture, the methane production rate increased with increasing dilution rate. The maximum methane production rate of 750 mM d(-1) was observed at a dilution rate of 16.2 d(-1), which is impossible without immobilization of the cells in the sediment. Using scanning electron microscopy, we observed Methanosaeta-like filamentous microorganisms and Methanosarcina-like coccoid microorganisms in granule-like structures. The results demonstrated that marine sediment is not only a promising microbial resource of halophilic aceticlastic methanogens, but also a supporting material to fix aceticlastic methanogens in a fixed-bed reactor.

• 出版日期2016-1