Analysis of triterpene saponins in Semen Aesculi by high performance liquid chromatography-electrospray-time of flight-mass spectrometry

作者：Chen Jun-Hui^*; Li Wen-Long; Yang Bai-Juan; Frank Sen-Chun Lee; Wang Xiao-Ru; Yang Huang-Hao

来源：Chinese Journal of Analytical Chemistry, 2008, 36(3): 285-291.

• high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry
• Semen Aesculi
• saponins

A convenient and user-friendly method based on high performance liquid chromatography (HPLC) and positive ion electrospray-time of flight-mass spectrometry (ESI-TOF/MS) has been developed for analysis and characterization of triterpene saponlins in extract of Semen Aesculi. Samples were analyzed by a reversed-phase C, column using a binary eluent of acetonitrile and water (with 0.2% acetic acid) under gradient conditions. The [M + H](+) or [M + Na](+) ions of saponins in Semen Aesculi extract were observed by EST-TOF/MS on-line detection, and used to obtain accurate molecular weight and molecular formula information of saponins. Fourteen saponins in Semen Aesculi extract could be primary identified by accurate molecule weight information and fragmentation behavior obtained by collision induced dissociation experiment combined with literature review. This method has the advantages of simple operation, rapid measurement and accurate determination and it is a powerful toot for identification of saponins in Semen Aesculi.

• 出版日期2008-3
• 单位国家海洋局第一海洋研究所; 中国海洋大学; 厦门大学