Molecular cloning of HMW glutenin subunits from Roegneria kamoji (Poaceae: Triticeae)

作者:Lei, Ying-Xia; Yu, Kuai; Wang, Yi; Fan, Xing; Kang, Hou-Yang; Sha, Li-Na; Zhou, Yong-Hong; Zhang, Hai-Qin*
来源:Biologia, 2016, 71(4): 414-420.
DOI:10.1515/biolog-2016-0055

摘要

In the present study, The hexaploid species Roegneria kamoji (StStYYHH genomes) in Triticeae produced a single high molecular weight glutenin subunit (HMW-GS) 1St1.2 in its endosperm. 1St1.2 encoded 404 amino acid, which is shorter than previous reported HMW-GSs in Triticeae. The N-terminal domains of 1St1.2 resemble that of the y-type subunits, while their C-terminal domains are similar to the x-type subunits. We discovered many variation types of polypeptides in central repetitive domain. There also have amino acid mutations in the N-terminal domain. The results of phylogenetic analysis suggested that 1St1.2 might be expression from the St genome. Therefore, the results indicated that 1St1.2 is a novel HMW-GS variant or isoform, and the characterization of the subunit can enhance our understanding on the structural differentiation and evolutionary relationships of HMW-GSs in wheat and its wild relatives.

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