Multi-drug Resistance and Characteristic of Integrons in Shigella spp.Isolated from China

作者：Zhu JingYuan; Duan GuangCai^*; Yang HaiYan; Fan QingTang; Xi YuanLin

来源：Biomedical and Environmental Sciences, 2011, 24(1): 56-61.

DOI：10.3967/0895-3988.2011.01.007

• Resistance
• Shigella flexneri
• Integron
• Gene cassettes

Objective To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp.
Methods Ninety Shigella strains (83 5. flexneri and 7 5. sonnei) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.
Results High prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%) carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intl3 was detected in the tested isolates. The prevalence of intl2 was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P < 0.05). Gene cassettes dfrA17-aadA5, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.
Conclusion The class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.

• 出版日期2011-2
• 单位河南大学; 郑州大学