A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2

作者：Ge H^*; Si YZ; Wolffe AP

来源：Molecular Cell, 1998, 2(6): 751-759.

DOI：10.1016/S1097-2765(00)80290-7

Increasing evidence suggests that pre-mRNA splicing can take place cotranscriptionally in vivo. However, insight into how these two processes are linked has been lacking, Here, we describe that a novel transcriptional coactivator, p52, interacts not only with transcriptional activators and general transcription Factors to enhance activated transcription but also with the essential splicing factor ASF/SF2 both in vitro and in vivo to modulate ASF/SF2-mediated pre-mRNA splicing. Furthermore, immunofluorescence studies indicate that the majority of endogenous p52 is colocalized with ASF/SF2 in the nucleoplasm of HeLa cells. Together, these observations suggest that, in addition to functioning as a transcriptional coactivator, p52 may also act as an adaptor to coordinate pre-mRNA splicing and transcriptional activation of class ii genes.

• 出版日期1998-12
• 单位NIH