科研之友
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摘要
Good's syndrome (GS) is often accompanied by recurrent respiratory infections and chronic diarrhea. The main purpose was to evaluate the peripheral immune status of a GS patient after thymoma resection. Twenty healthy volunteers were recruited as healthy controls (HCs). Flow cytometry was applied to determine the proportions of circuiting CD4(+) T cells, CD8(+) T cells, gamma delta T cells, and regulatory T (Treg) cells in our GS patient. We also examined the proliferation capability of ex vivo CD4(+) T cells and detected the levels of cytokines interferon- (IFN-) gamma and interleukin-17A secreted by ex vivo immune cells from this GS patient. Compared with healthy control subjects, this GS patient had fewer B cells, an inverted ratio of CD4(+)/CD8(+) cells, and more Treg cells in his peripheral blood. Additionally, the patient's V delta 2 T cell levels were significantly decreased despite having a normal percentage of gamma delta T cells. Ex vivo peripheral CD4(+) T cells from the patient showed insufficient proliferation and division potential as well as excessive expression of PD-1. Moreover, IFN-gamma was predominantly derived from CD8(+) T cells in this GS patient, rather than from CD4(+) T cells and gamma delta T cells. This GS patient had impaired T and B cell immunological alternations and cytokine disruptions after thymectomy. Detailed research should focus on therapies that can adjust the immune status in such patients for a better outcome.
- 出版日期2018
- 单位南京医科大学
