Development of SNP markers associated with growth-related genes of Pelodiscus sinensis

作者：Zeng, Dan; Li, Xiao; Wang, Xiao-qing^*; Xiong, Gang^*

来源：Conservation Genetics Resources, 2020, 12(1): 87-92.

DOI：10.1007/s12686-018-1065-5

• Pelodiscus sinensis
• Next generation sequencing
• Single nucleotide polymorphism (SNP)
• Amplification refractory mutation system PCR (ARMS-PCR)

The Chinese soft-shelled turtle (Pelodiscus sinensis) is one of the most important aquatic animals in China. The low growth rate is the main factor which hinders the development of the turtle's industry. Therefore, it is urgent to development available molecular marker associated with genetic variation in growth for P. sinensis. The aim of this study was to identify and validate SNPs from growth-related gene of P. sinensis. The Illumina Hiseq4000 sequencing was used to develop single nucleotide polymorphism (SNP) markers in P. sinensis. Among them, 38 SNPs from growth-related genes were randomly selected and 24 loci exhibited significant polymorphism in genotyping by ARMS-PCR. The observed and expected heterozygosity ranged from 0.0625 to 0.5208 and 0.0807 to 0.4974, respectively. Significant deviation from HWE was observed for 3 loci. These findings provide molecular basis and candidate markers for P. sinensis breeding.

• 出版日期2020-3
• 单位湖南农业大学